Cell online: November 30, 2011
Structural Basis for Promoter −10 Element Recognition by the Bacterial RNA Polymerase σ Subunit
Andrey Feklistov and Seth A. Darst
We determined crystal structures of σ domain 2 bound to single-stranded DNA bearing -10 element sequences. The structures, along with biochemical data, support a model where the recognition of the -10 element sequence drives initial promoter opening as the bases of the nontemplate strand are extruded from the DNA double-helix and captured by σ.